Charging Up

This video shows polymer cantilevers charging up in an electron microscope. At 2kV, charging is not a huge problem. But, at 5kV accelerating voltage, the cantilevers accumulate enough charge to get pulled down to the silicon substrate. From this video (sped up 6x), Jaz determined the need to use low accelerating voltages when trying to measure how much the cantilevers move because of laser illumination, so that the electron beam doesn’t interfere with the measurements.